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??With the progress of the alveolar surgical technique and the renewal of the equipment??at present??all of the clinically affected teeth can be extracted by conventional extraction techniques and surgical extraction techniques. However??there are still complications in some particular cases of anatomical location. In order to avoid the occurrence of complications??some special equipment and techniques have also been used for the extraction of some complex teeth. This article elaborated the work principle??the merit and shortcoming and the application scope of some special equipment and the technology in order to provide reference for the choice of tooth extraction equipment in different clinical cases.     

应用DNA探针检测蚊体内丝虫幼虫的研究

本文用人工合成的寡核苷酸探针，经斑点杂交法检测蚊体内马来丝虫幼虫。可测到丝虫和微丝蚴２ｎｇ的ＤＮＡ量，不与其它动物丝虫标本发生交叉反应。将单个蚊直接压于硝酸纤维素膜上进行检测，感染蚊中含有１条感染期幼虫就可出现阳性反应。将一组蚊虫在裂解液中研磨集体检测时，在２０只蚊虫中有１只感染蚊即可检出。显示该探针可用于马来丝虫地区的蚊媒监测。     

Diagnostic utility of automated indirect immunofluorescence compared to manual indirect immunofluorescence for anti-nuclear antibodies in patients with systemic rheumatic diseases: A systematic review and meta-analysis

Objective

This study aimed to review and compare the analytical and clinical performance of automated indirect immunofluorescence (AIIF) and manual indirect immunofluorescence (MIIF) as anti-nuclear antibody screening assays for patients with systemic rheumatic diseases (SRDs), such as systemic lupus erythematosus (SLE) and systemic sclerosis (SSc).

Hypertensive labeling: Does it have therapeutic implications?

Summary Screening for hypertension within the proper setting potentially leads to the detection of numerous (mild) hypertensives, some of whom in due time may well qualify for treatment. One disadvantage of the procedure appears to be that hitherto unwitting hypertensives may develop some adverse psychological and physiological phenomena. Behavioral effects seem to be particularly obvious after screening on the worksite, where newly identified hypertensive employees tend to respond with increased absenteeism and allied symptoms of labeling. In the clinical setting, physiological studies in recently informed hypertensives have demonstrated a series of signs of increased sympathetic activity: heart rate, cardiac index, circulating catecholamines, and renin, and even blood pressure itself, tend to be higher in aware vs. unaware young hypertensives. Although some correlations between psychological and physiological sequelae have been observed, the true nature of such links is far from clear. Simple anxiety does not seem to be the main mechanism. Pending further research, the question is discussed as to whether these phenomena are harmful enough to warrant a need for counseling or treatment.     

人工全膝关节置换术截骨方法改良及截骨测量

目的：对人工全膝关节置换术股骨截骨方法进行了改良，通过截骨厚度的测量和临床随访对改良截骨方法的手术效果进行评估。方法：采用改良股骨截骨模板，股骨后髁截骨厚度增加3mm。对81例行人工全膝关节置换术患者术中的各项截骨厚度进行测量，分为膝内翻和膝外翻两组，对截骨厚度和假体厚度进行比较分析。术后平均随访27．1个月。结果：81例患者的屈膝间隙和伸膝间隙平均截骨厚度均小于假体厚度。膝内翻和膝外翻组间除股骨后内侧髁截骨厚度外，各项测量值间均存在显著差异。膝内翻组内侧屈伸膝间隙截骨厚度和假体厚度差异最大；膝外翻组外侧屈伸膝间隙截骨厚度和假体厚度差异最大。术后关节活动度良好，仅7．4％病例存在轻度屈膝不稳，无中、重度不稳。结论：本文对股骨截骨模板的改良是可行的，增加3mm的截骨厚度不会出现过度截骨和屈膝不稳的情况。     

重组人P53融合蛋白抗肝癌细胞的实验研究

研究蛋白传导域(PTD)与人野生型P53(WtP53)融合表达的P53融合蛋白PTD-P53对HepG2的增殖抑制和凋亡的影响.用PTD-P53处理HepG2后,采用MTT法分析处理后细胞的生长增殖情况,流式细胞术(FCM)分析处理后细胞周期变化及脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测细胞凋亡情况.PTD-P53对:HepG2的生长有明显抑制作用.细胞周期也出现阻滞现象,细胞凋亡明显增加.PTD-P53有一定的抗肝癌细胞作用.     

丙型肝炎抗体的检测与HCVRNA定量及ALT的关系探讨

目的比较不同实验诊断方法在丙型肝炎(丙肝)抗体检测中的应用价值,并探讨HCV RNA定量与ALT指标之间的关系。方法对105例增强化学发光法(CIA)检测丙肝抗体初筛结果呈阳性(S/Co>1)的标本,分别采用酶联免疫吸附试验(ELISA)和荧光定量聚合酶链反应(RFQ-RT-PCR)进行检测,并对可疑结果(①CIA检测结果S/Co在1～8之间的标本,②ELISA检测结果呈阴性的标本)采用HCV RIBA3.0进行确认;应用OLYMPUS AU-5400全自动生化仪及其生化检测试剂检测全部标本ALT指标。结果ELISA及RFQ-RT-PCR方法检测样本阳性率分别为93.33%和70.48%;对可疑标本经HCV RIBA3.0确认试验显示,2例结果呈阳性(分别为NS3、HCV-C和NS3、NS4阳性),1例为阴性,4例仍为可疑(其中1例HCV RNA>103copies/ml);HCV RNA含量呈阳性的样本中,ALT异常率与HCV RNA含量间呈正相关(r=0.96,P<0.01),而ALT数值的变化与HCV RNA含量并无相关性(r=0.19,P>0.05)。结论第三代ELISA诊断试剂盒检测丙肝抗体同样具有较高的灵敏度,2种检测方法均可能存在假阳性或假阴性情况;RFQ-RT-PCR检测结果阳性率低于CIA与ELISA检测结果;在临床诊断中丙肝抗体的检测应与荧光定量聚合酶链反应一起使用,以提高临床丙肝诊断的准确率。     

Pressure oscillation amplitude after interruption of tidal breathing as an index of change in airway mechanics in preschool children

Bronchodilator reversibility testing using change in airway resistance during interruption (Rint) is feasible in preschool children. Analysis of postocclusion oscillations of the mouth pressure-time transient (Pmo(t)), recorded during airflow interruption, may offer an alternative index of change in airway mechanics. We analyzed Pmo(t) oscillation amplitude in three different ways: 1) difference between the first relative maximum and minimum (AMxMn); 2) detection of the dominant frequency using Fourier analysis (AFS); and 3) curve-fitting based on a mathematical model (ACurv). In 25 asymptomatic asthmatic children, aged 2.5-5.6 years, who had undertaken reversibility testing, the correlation coefficients between baseline Rint and amplitude were: AMxMn r = -0.84, AFS r = -0.82, ACurv r = -0.84. The coefficient of variation (CoV) of readings contributing to baseline Rint measurement, as median (range), was 12% (5-24%), which was not significantly different from AFS or ACurv (P > 0.05). All parameters were significantly different postbronchodilator (P < 0.001). Using the sensitivity index, i.e., the change after intervention divided by the baseline standard deviation, ACurv was the most sensitive and Rint the least sensitive, with median (range) at 2.72 (-0.84 to 12.10) and 1.91 (-1.17 to 9.50), respectively (P = 0.005). Our results suggest that oscillation amplitude analysis may provide a sensitive index of change in airway mechanics in preschool children undertaking bronchodilator reversibility testing.     

Left ventricular function changes during pharmacological and physiological interventions and routine activities monitored in healthy volunteers by a portable radionuclide probe (VEST)

A miniaturized radionuclide cardiac probe incorporated in a semi-rigid plastic chest garment has made ambulatory left ventricular (LV) function evaluation possible, with gated nuclear data being stored on tape together with electrocardiographic data, for subsequent off-line processing.After red blood cell labelling with 555 MBq (15 mCi)^99mTc and standard gated blood pool imaging in 45° LAO, we performed a continuous monitoring of LV function in 20 healthy male volunteers (age range: 22–25 years), in resting control conditions as well as during activities (standing, walking, climbing stairs) and after interventions (isosorbide dinitrate intake, Trendelenburg position, inflation of cuffs around the thighs).VEST-monitoring proved to be a reliable method that gave reproducible results: changes of ejection (EF) in basal conditions were lower than 5% in 95% of the patients. Changes in LV function caused by daily activities were easily demonstrated. While standing effected no significant EF changes, walking and climbing increased EF by 6.9% (p<0.05) and 21.2% (p<0.05) respectively.Changes in LV volumes caused by alterations in venous return were also demonstrated. Compared with baseline, Trendelenburg increased end-diastolic volume (EDV) by 2.9% while isosorbide dinitrate and inflation of cuffs decreased it by 5.7% and 2.2% respectively.